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Getting the Most Out of Fruit in Beer: Jammiest Bit & Fruit of Many Uses

Good To Know

While I love the pure expression of malt, water, hops, microbes, barrel, and time... fruit can make a fantastic addition to a barrel-aged sour. From a "sales" standpoint it is also easier to explain the difference between beers with fruit, than those with subtle differences in grain bill or microbes. Most people know they like cherries, plums, and raspberries... they may not know they enjoy horse blanket, minerality, or rubbery notes. 

This is the third in a series of posts updating my thoughts from American Sour Beers ten years ago. The two beers featured below (Jammiest Bit and Fruit of Many Uses) were both part of the first shipment of the Sapwood Cellars Out of State Shipping Club. Memberships for that shipment are closed, but you can still sign-up now for the next shipment fall 2024 ($146/shipment including shipping). Bottles of both are still available at the tasting room!


Sourcing Fruit

I would almost always rather use fresh in-season local fruit over a puree, juice, freeze-dried, concentrate, and especially natural extract. Local produce tastes unique, meaning a beer that doesn't taste the same as one brewed on the other side of the country or by a larger brewery. You'll get a more complex character from having beer in contact with the stems, skins, pits etc. Working with farms, orchards, and vineyards "fits" the narrative of an artisanal product... making it look good on social media too! Not to mention our fruiting tanks (without conical and glycol jackets) work best with whole fruit rather than purees.


Talking directly to farms and orchards at farmer's markets is a great place to start. Tasting things, chatting about what sort of capacity (and excess) they might have. Sometimes you can get a good deal on seconds... but for me these often aren't worth it since it can take a lot of time and effort to sort them (going rotten before they are all ripe) and cut-out mold etc. 


We've used IQF (Individual Quick Frozen) fruits several times with great results. For smaller batches I've just gone to supermarkets, found a product I like the flavor of... and bought out a couple locations. Online specialty purveyors like Northwest Wild Foods have weird things you might not find locally, like honeyberries. For lager batches we've ordered from Coloma Frozen Foods, but don't do it regularly as refrigerated shipping is expensive. Recently we've been getting our raspberries from Twin Springs Fruit Farm (which freezes their own). Using high-quality frozen fruit is something great lambic breweries do, and it allows to extend the fruiting season so you don't have to have enough tanks for all of your fruit beer at once!


That said, not all fruits are available locally. You can certainly source whole fruits from a good local produce supplier (and we have), but purees have their place too... especially in "smoothie" type sour beers. That said, there is no magic bullet on sourcing them. We've used Oregon Fruit, Greenwood Associates, Kerr/Ingredion, Asceptic Fruit Purees, Hop Havoc, Boiron, FruitGuild, and Araza. None of them are "always good" and none of them are always bad. It's often about preference. For example, Oregon Pineapple is thin, closer to juice, great for an IPA where you'll drop out the solids. Araza Pineapple is much thicker, perfect for a smoothie sour. Both taste great. 


Frozen wine grapes are another great option, either juice or must. Again, I love working with local vineyards (Crow Vineyard and Winery has been especially nice to work with), but that isn't always an option! We've had good luck with Grapes for Wine and Wine Grapes Direct. 


The only concentrates I've enjoyed are "freeze" concentrates (the wild blueberry and raspberry from Greenwood Associates are great). I've disliked the flavor of all of the standard high-concentration "boiled" concentrates which end up tasting like caramel, and are so thick that they are difficult to mix with beer. Granted, after an early batch with concentrates from Kerr we really haven't tried any more. On the other hand, Kerr's NFC (Not From Concentrate) raspberry juice was terrific, so I suspect the issue was the concentration. 

Freeze-dried fruit can be a pretty good option for tropical fruits like mango. I've yet to find a mango puree that didn't taste cooked. The freeze-dried stuff tends to have a brighter "mango popsicle" flavor. It can be pricy, but there is a lot of flavor pound-for-pound. North Bay Trading generally seems to have the best bulk pricing. 


Processing

We have a chest freezer at the brewery so we can keg fruit when it is ready, but not necessarily have to go onto it immediately. Freezing the fruit also helps break the cell walls allowing better/quicker contact and refermentation. Just make sure to line your freezer with cardboard so the bags don't stick to the interior. That's all we do for berries. Freeze them, let them thaw in the tank, then transfer beer on the next day.

When it comes to larger fruits (e.g., stone fruit like peaches and nectarines) we'll manually quarter them and discard the seeds/pits. In an ideal world we'd selectively process and freeze the fruit on a flow basis as it reached peak ripeness.

I've never had good luck with fermented citrus, so for limes, lemons, oranges, and grapefruit we use the zest. We use a Starfrit Electric Rotato Express. It struggles a bit on really large grapefruits, and we go through a couple a year as they just aren't sturdy enough for a "production" environment. 


There is some science that cherry stems have glycosides that Brett can work on to free fruity aromatics... but in practice I find they add a "stemmy" flavor that reminds me of dried leaves. I like the pits though. Obviously nice to find a source that has your preferred processing so you don't have to do it!

However you process the fruit, purge the tank with CO2 thoroughly before transferring beer in. This will help ensure the brightest, freshest, fruit expression possible!


Reyeasting

We usually repitch rehydrated (with StartUp/GoFerm) wine yeast along with the fruit to ensure a rapid refermentation, scavenge oxygen, and enhance the fruit character. While our sour beer production area has "light" HVAC, we don't have jackets for our fruiting totes (Flextanks). As a result, we'll change our yeast depending on the seasonal temperature. Usually using more heat-tolerant red wine strains in the summer, and cool-loving whites in the winter. 

We also add a small amount (5-7 PPM) of hop extract to prevent additional acidification if the beer is already sour enough for our tastes. See my article on hopping sours for more details.


Fruit Contact Time

I've slowly come to be an advocate for relatively short contact time, enough to ferment out the sugars, but not much more. This is especially true of raspberries (which develop a "seedy" flavor from extended contact). I've also heard strawberries as a quick-contact to reduce the phenolic "plastic" flavor they can develop. Although I've also read that can be varietal specific, and others claim freeze-drying can help mitigate. 

If I'm aging a beer on raspberries and another fruit that I prefer longer contact, I'll do that separately in two totes. That way we can keg off the raspberry after 10-14 days, while the cherries, wine grapes etc. have a little more contact time (1-2 months). They we blend them together. This could also be accomplished sequentially by racking the beer off of the raspberries and then onto the cherries. 


Separating Fruit and Beer

Our Flextanks have stainless steel filters from Utah Biodiesel Supply fitted over the racking arms with stoppers. A false bottom would likely work even better, but with a slow transfer we haven't had many issues with whole/pieces of fruit. The lone one I remember causing havoc with chunks of frozen mango that totally disintegrated. 

Second Use Fruit

With all the time and effort of getting the fruit and processing it, we often try to get a second beer out of it. Second use fruit is more subtle, allowing a more "beer-forward" balance. You could likely get similar results from ~25% of the fruiting rate, but second use fruit is easier (and free)!


We'll often just push in a single keg of sour beer onto the fruit from a whole batch to "rinse" it. This gets a big fruit character and it's an easy way to make a unique one-off

We'll do a whole new batch onto especially high fruiting rate beers. For example when we did 4 lbs/gal of raspberries in Throwing Hearts with Other Half, we went onto the fruit with a sour red along with vanilla beans to make Galactic Swirl.



For Fruit of Many Uses, we racked the beer sequentially into each tote after a previous beer. Getting Chardonnay grapes (from Field Learning, our Bissell Brothers collab) before going into barrel, followed by raspberries, then cherries (both from Jammiest Bit), and white nectarines (Polite Company). 

We haven't tried it, but I know some brewers who will knock-out fresh wort onto spent fruit as a way to get fruit flavor along with a strong house culture. 

No matter your technique, be extra mindful of limiting oxygen exposure as you won't have refermentation to scavenge oxygen.

Jammiest Bit

Barrel #71 Golden Strong #3 (Pils, 2-row, Chit, Wheat malt, and Flaked Wheat to 1.056 with aged Celia and Lemondrop pellets). Primary fermentation with 58W3 and some microbes from a blend of older barrels that was primarily Yeast Bay Mélange (but also various dregs from Oxbow, Jester King, and Backacre). Then aged 17 months in a second-use Malbec barrel. The culture in the barrel itself was originally derived from a De Garde bottle. 

Barrel #36 Marylambic #7 (Weyermann Barke Pilsner, Flaked Wheat, and Chit to 1.044 with .5 lbs/bbl aged East Kent Goldings). Primary fermentation T58. Then aged 13 months in a third-use Pinot Noir barrel. The microbes in the oak were originally from dregs from two Floodlands bottles. 

Racked into two totes one with 150 lbs Twin Springs Raspberries and the other with 150 Baugher's Orchard Sour Cherries. Both frozen and thawed. Each received 10 g of HopSteiner Alpha Extract, and fresh 71B for refermentation. 


Tasting Notes

Smell - Mix of fresh berries, raspberries more than cherries. Not a hugely complex funky or "Brett-forward" beer, but there is a little lemon and hay Barrel character is hidden behind the fruit as well. 

Appearance - Crystal clear, brilliant red-purple. Light-pink head fizzles quickly, but stays as a thin covering.

Taste - Cherries come through more on the palate. Good fruit intensity, still really fresh/vibrant. Firm acidity, slight sharpness from the malic acid of the cherries? Again not an especially complex or funky beer, but it’s a showcase for good fruit. The Baugher's cherries without stems seems to have been a good choice. A bit sweet which lends a more Flemish Red lean rather than Lambic or Saison.

Mouthfeel - Medium-high carbonation. Medium-light body. 

Drinkability - It grows on me as I drink it and my palate gets used to the acidity.  Good blend of fruit, bright flavor, I just wish the base beer was a little more interesting. 

Changes for Next Time - Wouldn’t mind depitted cherries to make sure we are getting good extraction.

Fruit of Many Uses

Base Beer: Belgian Pale #3

78% Murphy & Rude Virginia Pilsner

18% Murphy & Rude Wheat Malt

4% Murphy & Rude Vienna Malt

OG 1.047

.5 lbs/bbl 5-year-old Australian Summer Hop Pellets

Primary Yeast Lalvin 71-B

Microbes from Chardonnay Grape tote (originally a Bissell Brothers house souring culture they sent for the collab).

Then aged in a third-use Cabernet Sauvignon (microbes originally from Modern Times House of Sand) and Barrel #41 fourth-use merlot barrel for 11 months (microbes in barrel included East Coast Yeast Senne Valley, Bokkereyder dregs, Mad Fermentationist Saison, Casey, and Afterthought dregs)

Racked sequentially onto 150 lbs Twin Springs Raspberries, 150 Baugher's Orchard Sour Cherries, and 250 Twin Springs White Jade Nectarines. 


Tasting Notes

Smell - The berry leads, more cherry than raspberry. Earthy hay, candied fruit salad. The nectarines and grapes don’t shine in the aroma, but they help to send it in a direction that isn’t one-note “berry.” Almost apricot brandy as it warms. I don’t taste anything off from the pits, seeds etc. 

Appearance - Carbonation seems a little low. A hard pour results in only a two-finger white head. A few bubbles rising through the pale body. Good clarity. 

Taste - Pleasantly tart lemony acidity, without being harsh. The nectarine comes through more distinctly on the palate. Finish is berry again, but light, bright, and juicy finish. Non bitterness.

Mouthfeel - Medium carbonation, could be spritzier. Smooth, no astringency. Light body, without being thin. 

Drinkability - Really complex with the different fruit notes coming in and out of awareness. 

Changes for Next Time - The Chardonnay is mostly lost, would be a better feature in a plain beer or maybe light dry hopping. 

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Yeast Autolysis and Yeast Bite in Beer Brewing

Good To Know
This week I take a look at yeast autolysis which is the decomposition of yeast, and how it affects beer flavor as your beer ages. Yeast Autolysis The word autolysis literally means self-destruction, and yeast autolysis is the final stage for a yeast cell life where the yeast cell wall literally bursts open and releases […]
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Are Thiols a Scam? Thiolized Yeast, Phantasm, and the Rise of Designer Beer

Good To Know
Thiols are the coolest thing in brewing hoppy beers since the invention of dry hopping. It seems like almost every collab Sapwood Cellars has been invited to brew over the last 18 months ends up "Thiolized": 3S4MP (Cosmic Punch IPA with Phantasm and Nelson Sauvin at Fidens), Cone Concentrate (Cosmic Punch DIPA with Simcoe at Other Half DC), Celestial Paradox (London Tropics IPA with Strata/Galaxy/Citra/Simcoe at Toppling Goliath), Yasokeee (Helio Gazer DIPA with Peacherine, Hydra, and Phantasm at Cushwa), and most recently Heisenberg (Helio Gazer DIPA with Galaxy/Nelson at Commonwealth).

So why all of the excitement from our fellow craft brewers? Obviously many of the collab requests are due to Scott's writing about and advocacy for thiols, but are they just something new that will be passé soon, or are these strains here to stay?


What Are Thiols?

Thiols are sulfur-containing compounds that are often potent aromatics. The ones brewers are excited about are tropical, winey, and citrusy, while other thiols are intensely unpleasant with aromas of garlic or rotten eggs... which is why the thiol mercaptan is added to natural gas to alert people to leaks. The "skunky" aroma of light-struck beer is also 3-methyl-2-butene-1-thiol.


Unlike many other beer aromatics that require concentrations in the ppm (parts per million) or ppb (parts per billion) many thiols have an aroma threshold in the range of 5-70 ppt (parts per trillion). This means that it doesn't take much of them to be apparent, but also means that it doesn't require "high" concentrations to become dominant. 


In terms of positive beer and wine aromatics, the thiols that get the most attention are 4MMP, 3MH, 3MHA, and 3S4MP. These have perceptions that range from passionfruit, to grapefruit, to rhubarb. These are the intense aromatics that give New Zealand Sauvignon Blanc wines their distinct aromas and are found free at low-levels in many New Zealand hops (as well as some other varieties from around the world).


Most of the thiols found in hops, malt, and other botanicals are bound and thus not active aromatically. Enzymes are required to free them. There are wine strains available capable of this, but getting those genes into brewer's yeast requires more work. 



Where Do Thiols Come From?

Bound thiols are found in both malt and hops, but levels vary widely. The bond in need of breaking comes in two "flavors" Cysteinylated (Cys) and Glutathionylated (Glu). The vast majority (90%+) in both malt and hops is Glu. The IRC7 gene in certain wine strains and Omega's Cosmic Punch can only work on the less common Cys. As a result, mash hops are most potentially beneficial for Cosmic Punch, as the enzymes in the mash (especially in the acid-protein rest temperature range) can help convert Glu to Cys. Luckily some less expensive hop varieties have the highest levels of bound thiols. We've used Saaz, Cascade, and Calypso with good results.


The more intense strains like Berkeley Yeast Tropics line and Omega's Helio Gazer, Star Party, and Lunar Crush can simply be added to a standard recipe with or without whirlpool hops. Rather than having more copies of the IRC7 gene, they have a wholly different gene which can free Glu-thiols directly. The IRC7 gene in Cosmic Punch is "sourced" from yeast, while patB Omega uses in the more assertive strains is from a bacteria (if transgenic CRISPR/Cas9 gene modification is a step too far for you). We fermented a kettle sour which only had a small dose of hexalone (isomerized hop extract for head retention) with London Tropics. The result was intensely passion-fruity, so much so that it could almost pass a fruit beer. 


GM (genetically modified) yeast strains aren't allowed in commercial beers in many countries (e.g., Canada, New Zealand). As a result there are labs working with wild isolates capable of freeing thiols for co-fermentations (e.g., CHR Hansen - it is primarily marketed for NA beers) and breeding strains with heightened thiol freeing capabilities (e.g., Escarpment). Omega initially worked on yeast breeding between English ale and a wine strain capable of freeing thiols... our trials with it (Designer Baby) were interesting, but had too much of the wine strain's idiosyncrasies present (poor flocculation especially). 



Pros & Cons of Thiol-Expressing Yeast

Pros:
-Intense aromatics that are otherwise impossible to achieve from hops, malt, and yeast
-Thiols have incredibly low aroma thresholds... measured in ppt (parts per trillion)
-Thiols are "free" no expensive hops or fruit required
-Thiols may help increase shelf-life by scavenging oxygen

Cons:
-Higher perceived "sulfur" aromatics are frequent in thiol-freeing strains
-Some brewers/consumers/countries prefer to avoid GM ingredients
-Thiols can be "one note"... is it really that different from adding a jar of passion fruit extract?
-Repitching the yeast doesn't make as much sense if you also want to brew English Ales, Porters/Stouts etc. 



More isn't (always) Better

Initially there was a focus on maximizing thiol concentration. This could include colder fermentation temperatures, mash hops, and engineering strains with more assertive genes. Like most aspects of brewing (or cooking) maximizing a single flavor compound doesn't usually result in the best overall flavor or balance. 


Thiols aren't typically a "primary" aromatic in beer, so a beer with over-the-top thiol concentration without anything to play off of can taste artificial. On the other hand, some of our heavily dry hopped DIPAs have tested at over 100X the flavor threshold for 3MH and still weren't the primary aroma thanks to competition from the "traditional" hop aromatics. From those test, I wouldn't worry about dry hopping removing all of the thiols.


As a general rule, I'd suggest using a more restrained approach to thiols in lighter/cleaner/simpler beers. It doesn't take much to add a unique twist to a lager or American wheat, where a double-dry-hopped DIPA or fruit beer may benefit from a much higher amount. In the end it's about your palate and goals for a beer. 


In my experience expressing thiols doesn't make every hoppy beer better. They add a distinct note that can greatly enhance the perception of passionfruit-type aromatics. That is a wonderful contribution when you are leaning into those flavors, but can be distracting or muddle other flavors. For example, I love the "mango popsicle" aroma of great Simcoe. However, with one of the intense strains like London Tropics or Helio Gazer in an all-Simcoe beer can become more generically "tropical" rather than varietal "mango." On the other hand, when dry hopping with passion-fruity Galaxy, or brewing with actual passionfruit the thiol note helps to enhance the existing aromatics. 



Phantasm

Hand-in-hand with thiol-expressing yeast goes Phantasm. It is essentially the dried and powdered remnants of the highest-thiol New Zealand Sauvignon Blanc grapes. As a result it comes with a high price-tag of ~$35/lb. It really doesn't smell like much before fermentation. Added to the whirlpool it adds a huge amount of bound thiols for the yeast to work on. 


During fermentation I detect a distinct "white grape" flavor and aroma that I don't get from other thiol-expressing ferments that rely on grain and hops alone for bound precursors. A lot of this drops out with the yeast however and the finished beers are rarely as distinct. To my palate the added expense is hard to justify in a highly-dry-hopped beer where the thiols are competing against other big aromatics. Unless you lean into those aromatics with a hop like Nelson Sauvin. I like Phantasm best when it is paired with actual white wine grapes, or in a simple base where it can star. 


We haven't used Berkely Yeast's "Thiol Boost" additive yet... but I'm just not that excited about a 15X increase on the already intense London Tropics level of 3MH. I'd be interested in herbs or additives that could push other unique thiol aromatics that we aren't getting from grain/hops. 



My Favorite Thiolized Batches

At Sapwood Cellars, we've brewed a few dozen batches between Cosmic Punch, London Tropics, Lunar Crush, and experimental isolates. I've enjoyed most of them, but a handful stand-out as beers I loved!


Cosmic Rings: Galaxy and Citra is one of our favorite combinations... but we have had a difficult time sourcing great Galaxy (that doesn't taste like honey roasted peanuts). This all-Citra double-dry-hopped pale ale starts with New Zealand Taiheke (Cascade) in the mash and kettle. It's one of our favorite whirlpool hops because it is low alpha acid and has a beautiful tropical aroma... not to mention a lower price compared to other New Zealand varieties like Nelson Sauvin and Riwaka. Then we ferment with Omega's Cosmic Punch which brings some tropical aromatics without becoming distracting or artificial. Finally we dry hop with Citra and Citra Cryo at a combined 3 lbs/bbl.


Tropical Pop: Kettle sour fermented with Berkeley Yeast London Tropics along with passion fruit and mango purees. Passion fruit is expensive, fermenting with London Tropics boosted the passion fruit aroma allowing us to use less without sacrificing the aroma intensity.


Field Learning: As a homebrewer I loved being able to dump a bottle of thiol-rich New Zealand Sauvignon Blanc into a keg of mixed-ferm saison. That wouldn't be legal commercially, but what we did for this collab with Bissell Brothers was to brew a restrained base with Hallertau Blanc in the mash and Phantasm in the whirlpool and age it in fresh Sauvignon Blanc barrels along with Bissell's house culture. After a year we added fresh Chardonnay grapes from Crow Vineyards here in Maryland. The tropical notes from the thiols survived barrel aging and gave the beer depth we wouldn't have gotten from grapes and barrel alone.



Takeaways

Are thiols a scam? No, but they also aren't an innovation that fundamentally changes what it takes to make a delicious beer. Consider a thiol-freeing yeast when additional passionfruit-type aromatics will enhance your beer. Don't worry about maximizing the thiols unless you they will be competing against other strong aromatics. 


I suspect that this is just the lead in to even more exotic genetically modified yeast strains (we have a pitch of Berkeley's Sunburst Chico which is modified to express high levels of pineapple-y ethyl butyrate). If you can produce a flavor compound without the cost, environmental impact, variability of growing it I suspect the economic pressures will be too great! That said, making delicious beers isn't about maximizing one or two compounds (in the same way that vanilla flavoring is inexpensive, but doesn't fully replace the depth and complexity of real vanilla beans). 




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Beer Design Case Study: Marzen

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This week I present a design case study for developing a Marzen beer recipe. Along the way I describe my thought processes as I work through creating and brewing a new recipe. Designing a Marzen Beer Before I start a new beer, I sit down and describe what I’m trying to accomplish. This beer was […]
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Small Craft Brewery Yeast Management

Good To Know

When it comes to brewing delicious beer, there are few aspects more important than the yeast. A healthy fermentation allows the malt, hops, and adjuncts to shine. Pitching the right amount of healthy cells helps ensure that the finished beer has the intended alcohol, expected residual sweetness, and appropriate yeast character.  

Over the last four years at Sapwood Cellars we've slowly improved our yeast handling. We've noticed improved fermentation consistency, and better tasting beers. Most of our process is excessive for a homebrewer, but it might give you some ideas!

Harvesting Yeast

We harvest yeast from moderate gravity beers when possible as these cells are less stressed and healthier as a result. Our general rhythm is to brew a pale ale with a fresh pitch, and harvest from that tank for an IPA and DIPA the following week. Once the pale ale fermentation is complete (repeated gravity readings, and no diacetyl or acetaldehyde sensory) we can and soft-crash to 56-58F (13-14C). Cold and dissolved CO2 encourage the yeast to settle out. Specific temperature and time are strain and tank dependent, but that works for most of the English-leaning strains we use (Boddington's, Conan, Whitbread, and the Thiolized-variants).

Once the beer has been cold for 24 hours, we attach a 1/2 bbl brink to the bottom of the tank and pasteurize through the line and brink with 180F (82C) water from our on-demand. 25 minutes hot ensures there aren't any stray microbes that will be passed onto the subsequent batches. After pushing out the water with CO2 pressure we spray the brink with cold water then pressurize it and the tank to ~10 PSI. 

We then dump about a gallon (4L) from the T until the yeast looks good (creamy, off-white) and then begin collecting into the brink. You don't need to dump a large volume of yeast. By keeping steady pressure on the tank and slowly releasing pressure on the brink through the valve at the top we ensure that the yeast won't come out of the cone too quickly (which could punch through pulling in more beer than yeast) and won't foam up in the brink. It takes 10-15 minutes to fill the brink. Usually we are able to collect 110-130 lbs (50-60 kg) before yeast starts coming out the top of the brink. 

We collect yeast before dry hopping to avoid having hops mixed in with the yeast. We also prefer the "less rough" flavor we achieve by dry hopping cold. If you dry hop early-mid fermentation and want to harvest, drop as much of the hops out as you can before crashing and harvesting.

Yeast Storage

Whenever possible we pitch within 72 hours of harvest. Larger yeast cultures generate more heat and thus tend to lose viability more rapidly. Store the yeast as cold as possible, which for us is ~36F (2C) in our walk-in. Ideally that would be closer to 32F (0C) to further slow its metabolism. Shake twice a day to dissipate hot-spots and vent down the pressure to knock-out CO2. If storing the yeast for more than a few days, attach a blow-off line to prevent pressure from building. 

There are studies about various additives for maintaining high yeast viability. We've added phosphate buffer to prevent a drastic pH drop. It's difficult to tell from a single data point, but viability dropped from 95% to 89% after a week of storage. We've seen closer to 10% reductions the handful of times we've stored yeast that long previously.  

We generally won't harvest and repitch beyond three generations (although recently we went to five). That's because with our limited number of tanks, variety of yeast strains, and canning schedule we'd eventually have to hold onto yeast for a couple of weeks before pitching or harvest from a strong beer. 

Determining Cell Count and Viability

There are plenty of successful brewers who pitch a standard weight by barrel/gravity, but knowing how many live cells you actually have is a great way to improve consistency. It's especially valuable if you use a variety of strains or want to bring in a new strain. Our harvests of the same strain can vary by as much as three times in terms of live cells per g of slurry (~.5-1.5 billion cells). The cost of all of the equipment required is ~$500, less than a single commercial 10 bbl yeast pitch from some labs. 

Start by shaking the brink to homogenize the culture. Then run a cup of yeast out, dump it (to avoid counting the cells packed around the port) and then pull a sample. The next step is to dilute the culture to a "workable" concentration - 1:100 for us. Too many cells packed together makes for a culture that is impossible/laborious to count, while too few raises the chances luck will throw-off the count. For a long time I diluted by volume, performing two sequential 10X dilutions with a micropipette. This had two drawbacks. First getting an accurate volume of yeast slurry is tricky because it is foamy and has small bits of trub that can plug-up the pipette. Second, we pitch by weight, so there was always some estimation when it came to converting the volume to a weight or the extra step of determining the physical density of the slurry by mixing with water in a graduated cylinder on a scale. What we do now is dilute by weight, which gives us cells per gram rather than cells per milliliter.

Our scale is accurate to .2 g, so weighing 1 g of yeast into 99 g of water has a ~20% margin of error. As a result I do 490 g of water with 5 g of the yeast slurry. This reduces the maximum margin of error to ~4%. After pouring the diluted culture back and forth to mix, I take 9.9 mL of the diluted culture with the micropipette and add .1 mL of a stock dye solution of Erythrosin B and phosphate buffer (1 g in 50mL of buffer). This results in a total dilution of 100X. You could go even further, a 10X dilution by weight (50 g yeast with 450 g of water) followed by a 10X dilution by volume (1 mL of the diluted culture with 8.9 mL water and .1 g of dye). Live cells are able to expel the Erythrosin B so they won't be stained, meaning any red yeast cells are dead. You can use a variety of other stains, but Erythrosin B is a food coloring and much safer to handle than methylene blue or trypan blue. Here's a post from Escarpmant Labs on using it inspired by my Tweet (which was in turn inspired by this).

Luckily the Boddingtons-type strain we use for most of our batches isn't "excessively" flocculent. When we fermented a run with Whitbread we ran into issues with the cells being too clumpy to count. Luckily BrewKaiser has a whole post on additions you can add to help. Phosphoric acid worked OK, but a local brewer suggested disodium EDTA, which I plan to buy before we do another run with a similar strain. 


Next, place a couple drops on the diluted culture a hemocytometer, apply the slide cover, and stick it under a microscope (we have an Omax). Count the live and dead cells in five squares (each made up of 25 small squares) - four corners, and center. This provides a large enough sample size to avoid undue randomness. A small tally counter helps keep track. The standard rule is to count cells touching the left and top lines, but not the right or bottom. Count connected cells as two only if the daughter cell is more than half the size of the mother. Then I plug the totals into Inland Island's Yeast Cell Count Calculator. Usually our harvests are 80-90% viable off a fresh pitch, and they tend to go up from there on subsequent generations (90-95%). If your viability isn't great it could either be that the yeast isn't getting enough nutrients/oxygen, your initial pitching rate was too high or low, or that you are waiting too long to harvest.  

There are automated solutions for yeast counting, but with some practice the whole processes will take less than 10 minutes.  



Pitching Yeast

To pitch, we attach the brink to a T inline during knock-out. With the brink on a scale we use CO2 to slowly push in the desired weight of yeast (calculated based on the cell count, wort gravity, and volume). We pitch during knock-out so the yeast mixes with the aerated wort as it goes into the fermentor. White Labs advocates using a pump to pitch their fresh yeast inline to achieve better mixing with the wort. Best practice is to do another cell count off the tank once knock-out is complete to validate your process (we did it a few times, but now trust our approach).

When we started brewing more double batches to fill our 20 bbl tanks, we were pitching enough cells for 20 bbls along with the first 10 bbls of wort. Our thought process was that the yeast wouldn't do much in the 3-4 hours before the second half of the wort went in. However, we found our fermentations were less reliable, often dragging towards terminal gravity, and the yeast from those batches had much lower viability than expected. Both of these issues improved significantly once we switched to pitching only enough cells for the initial knock-out volume. This allows for more growth and thus a higher proportion of younger yeast cells. 

Hopefully this overview of our process is helpful for someone starting a new craft brewery, or looking to take their yeast management to the next level. As with anything in brewing, the more variables you can track and control the more consistency you'll have in your results. Yeast management isn't a "fun" topic, but it is one of the simplest things a brewery can do to increase consistency, improve flavor, and save money!





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